Obesity affects far more than metabolism, altering immune activity, nerve structure, and tissue organization across multiple organs. To study these effects, researchers developed MouseMapper, an AI framework that analyzes whole-body imaging data in intact mice at cellular resolution. Using fluorescent labeling, tissue clearing, and light-sheet microscopy, the team mapped nerves and immune cells throughout the body. In obese mice, they found widespread inflammation and significant damage to the trigeminal nerve, which controls facial sensation. The affected mice also showed reduced sensory responses. Molecular analysis revealed obesity-related changes linked to nerve remodeling and inflammation, many of which were also found in human tissue. The findings suggest obesity-associated nerve damage is conserved across species and demonstrate MouseMapper’s potential for studying complex systemic diseases.